and Human Services (HHS)
EXPIRED
This Program Announcement expires on May 15, 2004, unless reissued.
THE ZEBRAFISH AS AN ANIMAL MODEL FOR DEVELOPMENT AND DISEASE RESEARCH
Release Date: May 16, 2001
PA NUMBER: PA-01-095
Trans-NIH Zebrafish Coordinating Committee
(http://www.nih.gov/science/models/zebrafish/)
National Institute of Child Health and Human Development
(NICHD, http://www.nichd.nih.gov/)
National Cancer Institute
(NCI, http://www.nci.nih.gov/)
National Center for Research Resources
(NCRR, http://www.ncrr.nih.gov/)
National Eye Institute
(NEI, http://www.nei.nih.gov/)
National Human Genome Research Institute
(NHGRI, http://www.nhgri.nih.gov/)
National Institute on Aging
(NIA, http://www.nih.gov/nia/)
National Institute on Alcohol Abuse and Alcoholism
(NIAAA, http://www.niaaa.nih.gov/)
National Institute of Arthritis and Musculoskeletal and Skin Diseases
(NIAMS, http://www.nih.gov/niams/)
National Institute on Deafness and Other Communication Disorders
(NIDCD, http://www.nidcd.nih.gov/)
National Institute of Dental and Craniofacial Research
(NIDCR, http://www.nidr.nih.gov/)
National Institute of Diabetes and Digestive and Kidney Diseases
(NIDDK, http://www.niddk.nih.gov/)
National Institute on Drug Abuse
(NIDA, http://www.nida.nih.gov/)
National Institute of Environmental Health Sciences
(NIEHS, http://www.niehs.nih.gov/)
National Institute of General Medical Sciences
(NIGMS, http://www.nigms.nih.gov/)
National Institute of Mental Health
(NIMH, http://www.nimh.nih.gov/)
National Institute of Neurological Disorders and Stroke
(NINDS, http://www.ninds.nih.gov/)
THIS PA USES THE MODULAR GRANT AND JUST-IN-TIME CONCEPTS. IT INCLUDES
DETAILED MODIFICATIONS TO STANDARD APPLICATION INSTRUCTIONS THAT MUST BE USED
WHEN PREPARING APPLICATIONS IN RESPONSE TO THIS PA.
This Program Announcement replaces PA-98-074.
PURPOSE
The purpose of this Program Announcement (PA) is to solicit applications as
part of a National Institutes of Health (NIH) initiative to increase our
support of the zebrafish as an animal model for development, organ formation,
behavior, aging, and disease research. This PA is a reissuance of PA-98-074,
which was published in the NIH Guide for Grants and Contracts, Vol. 5, No. 22
on May 21, 1998. This effort stems from an NIH initiative with participation
of the Institutes and Centers listed above, working though the Trans-NIH
Zebrafish Coordinating Committee (TZCC,
http://www.nih.gov/science/models/zebrafish/) under the co-chairmanship of
NICHD and NIDDK. Since its formation in 1997, the committee has played an
active role as an advocate for the zebrafish as an important model for
development, organ formation, behavior, aging, and disease research.
HEALTHY PEOPLE 2010
The Public Health Service (PHS) is committed to achieving the health
promotion and disease prevention objectives of "Healthy People 2010," a PHS-
led national activity for setting priority areas. This PA is related to one
or more of the priority areas. Potential applicants may obtain "Healthy
People 2010" at http://www.health.gov/healthypeople/.
ELIGIBILITY REQUIREMENTS
Applications may be submitted by domestic and foreign, for-profit and non-
profit organizations, public and private, such as universities, colleges,
hospitals, laboratories, units of State and local governments, and eligible
agencies of the Federal government. Racial/ethnic minority individuals,
women, and persons with disabilities are encouraged to apply as Principal
Investigators. Applications from new investigators are particularly
encouraged.
MECHANISM OF SUPPORT
This PA will use the National Institutes of Health (NIH) individual research
project grant (R01) award mechanism. Because the nature and scope of the
research proposed in response to this PA may vary, it is anticipated that the
size of awards will also vary. Responsibility for the planning, direction,
and execution of the proposed project will be solely that of the applicant.
Although this PA is the result of a trans-NIH initiative, awards will be made
through the Institute or Center whose mission is most closely related to the
proposed work. Through TZCC, each funding component will share with the
other committee members findings of research supported as a result of this
PA. All investigators funded under this initiative will be expected to work
together cooperatively so that the information learned will be of maximum
usefulness to the community.
For all competing applications requesting up to $250,000 direct costs per
year, specific application instructions have been modified to reflect
"MODULAR GRANT" and "JUST-IN-TIME" streamlining efforts being examined by
NIH. Complete and detailed instructions and information on Modular Grant
applications can be found at
http://grants.nih.gov/grants/funding/modular/modular.htm. Applications that
request more than $250,000 in any year must use the standard PHS 398 (rev.
4/98) application instructions.
RESEARCH OBJECTIVES
Background
The TZCC continues to play an active role as an advocate for the zebrafish
model. The committee’s initial efforts resulted in RFA DK-98-006, entitled
Genomic Resources for the Zebrafish, and continued with a Program
Announcement, PA-98-074, entitled The Zebrafish as an Animal Model for
Development and Disease Research. On May 10-11, 1999, the TZCC sponsored a
workshop entitled Genomic and Genetic Tools for the Zebrafish. At this
workshop, which expanded upon the Non-mammalian Models Workshop held on
February 16-17, 1999, zebrafish researchers were asked to help prioritize the
short- and long-term needs of the community. One result of the workshop was
the recommendation that more genetic screens in the zebrafish need to be
supported by the NIH. RFA-HD-00-004, entitled Mutagenesis
Screens/Phenotyping Tools for Zebrafish, addressed, in part, this and some
of the other recommendations made by workshop participants. Due to the
response of the community to this RFA, a follow-up PA (PA-01-070,
http://grants.nih.gov/grants/guide/pa-files/PA-01-070.html) was recently
released to provide an umbrella under which to continue NIH efforts to
support mutagenesis screening and development of phenotyping tools for
zebrafish. Since there has a been a continued interest in the further
development of this animal model, the original Program Announcement, PA-98-
074, The Zebrafish as an Animal Model for Development and Disease Research,
is being reissued here under the same title.
Vertebrate development has been characterized extensively using the methods of
classical embryology, molecular biology, and biochemistry. However, mutational
analysis in vertebrates has lagged behind such investigations in invertebrates.
As experimentation in Drosophila melanogaster and Caenorhabditis elegans has
established, mutational studies are a powerful tool to determine the events that
result in patterning and morphogenesis of the embryo. When combined with genetic
combinatorial analyses, mutational analyses can identify specific genes that act
during embryonic development, provide insight into how they function, and clarify
the pathways in which they participate. Studies that compare results from these
invertebrate systems with those obtained in vertebrates have established that
there is remarkable evolutionary conservation in the genetic programs that
determine embryo formation, including such early patterning events as formation of
the embryonic axes, but also including later events such as development of eye,
heart, and other organs. Although invertebrate systems are extremely powerful and
numerous aspects of development are conserved, many aspects of patterning and
morphogenesis of the vertebrate embryo are distinct and cannot be studied in
invertebrates. The vertebrate embryo has many features not present in other
models, including the substantially different organization and greater complexity
of the nervous system, and the fact that some vertebrate organs have no clear
cognates in the simpler invertebrates. Thus, understanding human development will
require application of experimental approaches to the formation of the vertebrate
embryo. Some assessment of mutations that affect development has been possible in
the mouse, but the mouse embryo is inaccessible in utero throughout much of its
development. Consequently, mutational studies in this species have been limited
largely to defects in post-natal maturation. While reverse genetics (e.g., knock-
outs) have been useful in the mouse model, the substantial costs of maintaining
large mouse colonies have limited the applicability of forward genetic approaches,
which will have a profound impact on our understanding of development.
As a vertebrate, the zebrafish, Danio rerio, is more closely related to humans
than are yeast, worms or flies. It has a number of valuable features as a model
organism for study of vertebrate development. Many features of zebrafish
development have been characterized, including early embryonic patterning, early
development of the nervous system, and aspects of cell fate and lineage
determination. The embryos are transparent and accessible throughout development.
In live embryos, the same specific cell or even cellular processes can, in many
cases, be identified from individual to individual, affording a high level of
precision in characterizing the effect of developmental or genetic perturbation.
Techniques for ablation and transplantation of individual cells have been used to
explore questions about induction and cell fate, and continue to be refined.
There are also a growing number of molecular markers to facilitate developmental
studies. Because of their relatively short reproductive cycle, the large number
of progeny that can be produced, and the relatively small space needed to maintain
large numbers of offspring, the zebrafish is an efficient vertebrate model system
for genetic analysis. It is possible to generate haploid progeny, which are
viable to the point where many recessive embryonic phenotypes can be identified,
and also homozygous diploid progeny that carry only maternal (or paternal) genetic
information. A genetic map of approximately two - three centimorgan resolution is
available, and mutations can be readily placed on the map. Positional cloning of
genes identified by mutation has recently been accomplished. Finally, there are
several promising methods for transformation and insertional mutagenesis which are
now being developed.
A number of mutagenesis screens have been performed to date and the transparent
embryos examined for defects in overall embryonic pattern, morphogenesis or organ
formation. These screens have identified a substantial number of mutations that
affect the formation of organ systems, including defects in the nervous system,
skeletal muscle, craniofacial region, kidney and endocrine organs, cardiovascular
and gastrointestinal systems, and the sensory cells of lateral line systems, which
are important to auditory and vestibular function. For most of these mutations,
the gene defect has not yet been identified. It is likely that many of these
mutations affect genes relevant to human development and aging and disease
processes such as neurodegeneration and cancer. The zebrafish offers the
opportunity of using classical genetics to define gene functions.
Scope
The NIH is interested in helping to develop and support tools to improve the
ability of model organisms to elucidate the genetic and molecular mechanisms that
regulate normal and abnormal biological processes. The zebrafish is one such
model that holds tremendous promise to increase our understanding of vertebrate
biology. The principle objective of this PA is to continue to promote the
zebrafish as an animal model for the study of development, organ formation,
behavior, aging, and disease research. Applications for investigator-initiated
research projects that will use state-of-the-art methods, and that will develop
any necessary new technologies to address well-defined hypothesis-driven research
questions will be appropriate for this PA. The goals of this PA are to encourage
new and innovative research and approaches using the zebrafish to identify the
genes and elucidate the molecular and genetic mechanisms responsible for normal
and defective development, organ formation, behavior, aging, and disease.
Each of the participating Institutes and Centers has specific interests in using
the zebrafish as a model system to better understand particular processes, organs
or diseases. In addition, some may be interested in supporting development of
methods, either general techniques or techniques that may particularly apply to
their areas of interest. The participating NIH Institutes and Centers have
provided a brief outline of their interests as they relate to the goals of this
PA. These brief mission statements are intended to give some understanding of the
breadth of the biomedical areas of interest in development of this model and are
listed below in alphabetical order. Participating Institutes have included
examples of research topics that are appropriate for this PA, however, they are
not to be considered as exclusive or limiting.
Institute and Center Statements of Interest
NCI: Generation and study of zebrafish models to identify and place genes in
functional pathways that affect growth and development, in particular,
genes/pathways that, when altered, result in uncontrolled or cancerous
growth. Identification of key sites within these pathways that could be
exploited for cancer therapeutic discovery purposes.
NCRR: The NCRR supports research projects that broaden the utility of the
zebrafish model for cross-cutting biomedical research that is not encompassed
within a single NIH Institute or Center. Interests include, but are not
limited to, development of new methods for mutagenesis and/or phenotypic
characterization that would be of use in research on a wide range of diseases
or organs, particularly if these methods could be applied to other animal
models as well as the zebrafish.
NEI: Fundamental mechanisms underlying all aspects of eye development,
function, and disease, including development of the retina and lens, optic
nerve axon guidance, and the neural circuitry producing eye movements and
oculomotor behaviors.
NHGRI: Proposals for the development of high throughput, widely applicable
technologies or methodologies to examine gene function on a genomic scale.
This could include initial development of high throughput or large-scale
methods for examining gene expression, development of tools for comprehensive
mutational analysis, or genome-scale identification of regulatory regions.
NIA: Basic research on the genetic and molecular basis of aging and
longevity. Generation and analysis of late-age onset or long-lived mutants
that can be used to identify, clone, and characterize genes involved in
normal and pathological aging. Cellular and molecular function of genes
expressed, for example, in the aging nervous system, cardiovascular, immune,
and musculoskeletal systems. Such genes include, but are not limited to,
those involved in neurodegenerative disorders, neuroplasticity, cell death,
damage and repair of DNA and proteins, and oxidative metabolism, and
maintenance of differentiated cell function.
NIAAA: Mechanistic studies of ethanol-induced teratogenesis, behavioral
impairments, and organ damage.
NIAMS: Mutations that have the potential to illuminate the development and
function of the vertebrate musculoskeletal system and skin. The
musculoskeletal system includes muscle, bone, articulated joints, cartilage,
tendon, and ligament. Priority will be given to the establishment of
collaborations between investigators with expertise in the zebrafish and
investigators with expertise in the musculoskeletal systems and skin of
mammals and humans.
NICHD: Identification, cloning, and characterization of the genes important
in normal development as well as those mutant genes that cause developmental
defects. Elucidation of the cellular, biochemical, molecular, and genetic
mechanisms underlying normal and defective development. This includes, but
is not limited to, the study of general mechanisms of pattern formation and
cell lineage, neural crest development, cell specification, differentiation,
migration, and fate in early development of many organs/systems such as limb,
nervous system, immune system, and heart.
NIDA: Identification of mechanisms underlying tolerance, sensitization, and
addiction to drugs of abuse such as nicotine, amphetamine, cocaine, opiates,
barbiturates, and hallucinogens. Identification of genetic suppressors and
enhancers of the teratological effects of drugs of abuse on behavior and the
nervous system. Processes involved in the development of brain regions
mediating the hedonic properties of drugs of abuse.
NIDCD: Identification and cloning of genes involved in the normal and
disordered development of hearing, balance, smell, and taste sensory systems.
Elucidation of the cellular, molecular, and biochemical mechanisms governing
the proliferative, plastic, and regenerative capacities of these sensory
cells and tissues.
NIDDK: Research on diabetes, particularly studies on pancreatic beta cell
function and development, obesity and mechanisms underlying satiety, other
endocrine and metabolic diseases, hematologic disorders, and diseases of the
digestive system, liver, kidney, and urinary tract. Studies aiming to
clarify the cellular and molecular events that dictate tissue and organ
formation in all these systems are considered of relevance. These studies
could include, but need not be limited to, studies to develop cell lines from
any of the tissues or organs of interest, studies to characterize normal or
abnormal function of tissues or organs of interest, methods to screen and
identify additional mutations in these systems, studies to define the
molecular mechanisms that dictate cell-specific gene expression in relevant
cell types.
NIDCR: All aspects of normal and abnormal craniofacial development,
including genetics, complex origins of craniofacial disorders, cell lineages
and differentiation, cell signaling and gene regulation, embryonic
patterning, imaging, biomimetics, and new technologies for high-throughput
genetic and protein screens.
NIEHS: Studies to examine the mechanism whereby environmental factors/agents
alter any aspect of development. This includes the screening for mutants that
ameliorate the toxicity of environmental agents, and the subsequent
identification and characterization of the genes and pathways involved in
their action. Characterization of the interactions among genetics,
environmental agents, and time during development that lead to structural or
functional abnormalities. Studies to examine the mechanistic pathways
involved in developmental exposure to environmental agents and subsequent
increased susceptibility to adult onset disease (developmental imprinting).
Development of a mechanistically based model for testing environmental agents
for developmental toxicity.
NIGMS: Basic biomedical research that addresses fundamental biological
mechanisms such as those that underlie gene regulation, chromosome
organization and mechanics, cell growth and differentiation, pattern
formation, sex determination, morphogenesis, cell cycle control, behavior,
the genetics of complex traits, and the application of mathematical models to
complex biological systems.
NIMH: Investigations that examine molecular, cellular, and biochemical bases
of genetic mutations affecting neurogenesis, biological rhythms, learning,
memory, and other cognitive functions and behaviors of the nervous system.
These studies include, but are not limited to, development of screening
methods for such mutations, identification, isolation, mapping, and
functional analyses of the genes underlying mutations.
NINDS: Research on the development and function of the nervous system.
Possible projects include, but are not limited to, studies of neurogenesis,
nervous system patterning, cell lineage, cell migration, programmed cell
death, axon pathfinding and regeneration, myelination, and cognitive, motor
and sensory function. Analyses of mutants that may serve as models for
neurological disorders are also encouraged.
The areas of interest listed above are not presented in any order of
priority, they are only examples of areas of research to consider.
Applications representing areas of interest to more than one Institute or
Center will be assigned to multiple Institutes or Centers for funding
consideration. Applicants are encouraged to propose work in other areas that
are related to the objectives and scope of this PA.
URLS IN NIH GRANT APPLICATIONS OR APPENDICES
All applications and proposals for NIH funding must be self-contained within
specified page limitations. Unless otherwise specified in an NIH
solicitation, Internet addresses (URLs) should not be used to provide
information necessary to the review because reviewers are under no obligation
to view the Internet sites. Reviewers are cautioned that their anonymity may
be compromised when they directly access an Internet site.
APPLICATION PROCEDURES
Applications are to be submitted on the grant application form PHS 398 (revised
4/98) and will be accepted at the standard application deadlines as indicated in
the application kit. These forms are available at most institutional offices of
sponsored research, on the Internet at
http://grants.nih.gov/grants/funding/phs398/phs398.html, and from the Division of
Extramural Outreach and Information Resources, National Institutes of Health, 6701
Rockledge Drive, MSC 7910, Bethesda, MD 20892-7910, telephone 301-710-0267, E-
mail: [email protected].
Applicants planning to submit an investigator-initiated new (type 1), competing
continuation (type 2), competing supplement, or any amended/revised version of the
preceding grant application types requesting $500,000 or more in direct costs for
any year are advised that they must contact the Institute program staff before
submitting the application, i.e., as plans for the study are being developed.
Furthermore, applicants must obtain agreement from program staff that the
Institute will accept the application for consideration for award. Finally,
applicants must identify, in a cover letter sent with the application, the program
staff member and Institute who agreed to accept assignment of the application.
This policy requires an applicant to obtain agreement for acceptance of both any
such application and any such subsequent amendment. Additional information about
this policy may be found in the NIH Guide for Grants and Contracts, March 20, 1998
at:
http://grants.nih.gov/grants/guide/notice-files/not98-030.html.
SPECIFIC INSTRUCTIONS FOR MODULAR GRANT APPLICATIONS
The modular grant concept establishes specific modules in which direct costs
may be requested as well as a maximum level for requested budgets. Only
limited budgetary information is required under this approach. The just-in-
time concept allows applicants to submit certain information only when there
is a possibility for an award. It is anticipated that these changes will
reduce the administrative burden for the applicants, reviewers, and Institute
staff.
Modular Grant applications will request direct costs in $25,000 modules, up
to a total direct cost request of $250,000 per year. (Applications that
request more than $250,000 direct costs in any year must follow the
traditional PHS398 application instructions.) The total direct costs must be
requested in accordance with the program guidelines and the modifications
made to the standard PHS 398 application instructions described below:
o FACE PAGE: Items 7a and 7b should be completed, indicating Direct Costs
(in $25,000 increments up to a maximum of $250,000) and Total Costs [Modular
Total Direct plus Facilities and Administrative (F&A) costs] for the initial
budget period. Items 8a and 8b should be completed indicating the Direct and
Total Costs for the entire proposed period of support.
o DETAILED BUDGET FOR THE INITIAL BUDGET PERIOD: Do not complete Form Page 4
of the PHS 398. It is not required and will not be accepted with the
application.
o BUDGET FOR THE ENTIRE PROPOSED PERIOD OF SUPPORT: Do not complete the
categorical budget table on Form Page 5 of the PHS 398. It is not required
and will not be accepted with the application.
o NARRATIVE BUDGET JUSTIFICATION: Prepare a Modular Grant Budget Narrative
page. (See http://grants.nih.gov/grants/funding/modular/modular.htm for
sample pages.) At the top of the page, enter the total direct costs
requested for each year. This is not a Form Page.
Under Personnel, list ALL project personnel, including their names, percent
of effort, and roles on the project. No individual salary information should
be provided. However, the applicant should use the NIH appropriation
language salary cap and the NIH policy for graduate student compensation in
developing the budget request.
For Consortium/Contractual costs, provide an estimate of total costs (direct
plus F & A) for each year, each rounded to the nearest $1,000. List the
individuals/organizations with whom consortium or contractual arrangements
have been made, the percent effort of all personnel, and the role on the
project. Indicate whether the collaborating institution is foreign or
domestic. The total cost for a consortium/contractual arrangement is included
in the overall requested modular direct cost amount. Include the Letter of
Intent to establish a consortium.
Provide an additional narrative budget justification for any variation in the
number of modules requested.
o BIOGRAPHICAL SKETCH: The Biographical Sketch provides information used by
reviewers in the assessment of each individual"s qualifications for a
specific role in the proposed project, as well as to evaluate the overall
qualifications of the research team. A biographical sketch is required for
all key personnel, following the instructions below. No more than three
pages may be used for each person. A sample biographical sketch may be
viewed at: (http://grants.nih.gov/grants/funding/modular/modular.htm).
-Complete the educational block at the top of the form page,
-List position(s) and any honors,
-Provide information, including overall goals and responsibilities, on
research projects ongoing or completed during the last three years,
-List selected peer-reviewed publications, with full citations.
o CHECKLIST: This page should be completed and submitted with the
application. If the F&A rate agreement has been established, indicate the
type of agreement and the date. All appropriate exclusions must be applied
in the calculation of the F&A costs for the initial budget period and all
future budget years.
o The applicant should provide the name and telephone number of the
individual to contact concerning fiscal and administrative issues if
additional information is necessary following the initial review.
Submission Instructions
The title and number of the program announcement must be typed on line 2 of
the face page of the application form and the YES box must be marked.
Submit a signed, typewritten original of the application, including the
Checklist, and five signed photocopies in one package to:
CENTER FOR SCIENTIFIC REVIEW
NATIONAL INSTITUTES OF HEALTH
6701 ROCKLEDGE DRIVE, ROOM 1040, MSC 7710
BETHESDA, MD 20892-7710
BETHESDA, MD 20817 (for express/courier service)
REVIEW CONSIDERATIONS
Applications will be assigned on the basis of established PHS referral guidelines.
Upon receipt, applications will be reviewed for completeness by the Center for
Scientific Review (CSR). Incomplete applications will be returned to the
applicant without further consideration. Applications will be evaluated for
scientific and technical merit by an appropriate scientific review group convened
in accordance with the standard NIH peer review procedures. As part of the
initial merit review, all applications will receive a written critique and undergo
a process in which only those applications deemed to have the highest scientific
merit, generally the top half of applications under review, will be discussed,
assigned a priority score, and receive a second level review by the appropriate
national advisory council or board.
Review Criteria
The goals of NIH-supported research are to advance our understanding of
biological systems, improve the control of disease, and enhance health. In
the written comments, reviewers will be asked to discuss the following
aspects of the application in order to judge the likelihood that the proposed
research will have a substantial impact on the pursuit of these goals. Each
of these criteria will be addressed and considered in assigning the overall
score, weighting them as appropriate for each application. Note that the
application does not need to be strong in all categories to be judged likely
to have major scientific impact and thus deserve a high priority score. For
example, an investigator may propose to carry out important work that by its
nature is not innovative, but is essential to move a field forward.
(1) Significance: Does this study address an important problem? If the aims
of the application are achieved, how will scientific knowledge be advanced?
What will be the effect of these studies on the concepts or methods that
drive this field?
(2) Approach: Are the conceptual framework, design, methods, and analyses
adequately developed, well-integrated, and appropriate to the aims of the
project? Does the applicant acknowledge potential problem areas and consider
alternative tactics?
(3) Innovation: Does the project employ novel concepts, approaches or
methods? Are the aims original and innovative? Does the project challenge
existing paradigms or develop new methodologies or technologies?
(4) Investigator: Is the investigator appropriately trained and well suited
to carry out this work? Is the work proposed appropriate to the experience
level of the Principal Investigator and other researchers (if any)?
(5) Environment: Does the scientific environment in which the work will be
done contribute to the probability of success? Do the proposed experiments
take advantage of unique features of the scientific environment or employ
useful collaborative arrangements? Is there evidence of institutional
support?
In accordance with NIH policy, all applications also will be reviewed with
respect to the following:
o The reasonableness of the proposed budget and duration in relation to the
proposed research.
o The adequacy of the proposed protection of human, animals, or the
environment, to the extent they may be adversely affected by the project
proposed in the application.
AWARD CRITERIA
Factors that will be used to make award decisions are as follows:
o Scientific and technical merit of the proposed project as determined by
peer review,
o Cost effectiveness of the proposed strategy,
o Promise of the proposed program to accomplish the goals of this PA and address
the needs of the participating Institutes and Centers as regards their interest in
the zebrafish as a model organism,
o Program priorities and program balance,
o Availability of funds.
INQUIRIES
Potential applicants are strongly encouraged to contact program staff with
any questions regarding the responsiveness of their proposed project to the
goals of this PA.
Written and telephone inquiries concerning this PA are encouraged. The
opportunity to clarify any issues or questions from potential applicants is
welcome. A complete listing of contacts for both programmatic and
fiscal/administrative inquiries may be found at:
http://www.nichd.nih.gov/PA/Zebrafish_animalModel.htm.
AUTHORITY AND REGULATIONS
This program is described in the Catalog of Federal Domestic Assistance Nos.
93.865, 93.396, 93.306, 93.867, 93.839, 93.172, 93.866, 93.273, 93.846,
93.173, 93.121, 93.847, 93.848, 93.849, 93.279, 93.113, 93.862, 93.242,
93.853. Awards are made under authorization of Sections 301 and 405 of the
Public Health Service Act as amended (42 USC 241 and 284) and administered
under NIH grants policies and Federal Regulations 42 CFR 52 and 45 CFR Parts
74 and 92. This program is not subject to the intergovernmental review
requirements of Executive Order 12372 or Health Systems Agency review.
The PHS strongly encourages all grant and contract recipients to provide a
smoke-free workplace and promote the non-use of all tobacco products. In
addition, Public Law 103-227, the Pro-Children Act of 1994, prohibits smoking
in certain facilities (or in some cases, and portion of a facility) in which
regular or routine education, library, day care, health care or early
childhood development services are provided to children. This is consistent
with the PHS mission to protect and advance the physical and mental health of
the American people.
Weekly TOC for this Announcement
NIH Funding Opportunities and Notices
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