Notice Number: NOT-OD-19-089

Key Dates
Release Date: March 14, 2019

PA-19-056
PAR-19-176
NOT-OD-19-081

Issued by
Division of Program Coordination, Planning and Strategic Initiatives, Office of Research Infrastructure Programs (ORIP)

Purpose

ORIP is issuing this Notice to highlight its interest in receiving grant applications for hypothesis-driven research projects through PA-19-056 NIH Research Project Grant (Parent R01 Clinical Trial Not Allowed) aimed at developing or improving cryogenic or other long-term preservation and revival methods of either Drosophila or zebrafish genetic stocks. Priority will be given to projects examining underlying mechanisms and processes critical for successful cryogenic or other long-term preservation and revival of Drosophila and zebrafish genetic stocks.

Areas of high priority include, but are not limited to, the following:

• Underlying molecular mechanism to improve efficiency, reliability, and reproducibility for genetic banking and revival of stocks, including vitrification methods;
• Molecular markers, such as for cell damage and molecular alterations including genetic drift, to evaluate the impact of cryogenic or other long-term preservation on revival, lifespan, and reproductive capabilities of genetic stocks;
• Extrinsic and/or intrinsic mechanisms underlying the extension of lifespan and reproductive lifespan, which are critical for reducing stock maintenance effort, including transgenic and dietary approaches (Drosophila only);
• Effect of dehydration on implantable gonad tissues and on the development of the germplasm (Drosophila only);
• Modulation of metabolic, signaling or molecular pathways to increase the efficiency of cryopreservation and survival of embryos (Zebrafish only).

Application and Submission Information

Submissions should indicate that they are in response to NOT-OD-19-089 in Field 4.b on the SF 424 form.

Principal investigators planning to submit applications on this topic are strongly encouraged to contact the scientific contacts listed in this Notice to be advised on the appropriateness of the intended research project, competitiveness of a potential application, and alignment with ORIP's program priorities.

Inquiries

Please direct all inquiries to:

Sige Zou, Ph.D.
Office of Research Infrastructure Programs (ORIP)
Telephone: 301-435-0749
Email: zous@mail.nih.gov

For inquiries specific to zebrafish research projects:

Miguel Contreras, Ph.D.
Office of Research Infrastructure Programs (ORIP)
Telephone: 301-594-9410
Email: contre1@mail.nih.gov