Whitehead Insitute may require documentation of acceptable ESCRO review (or equivalent) for the research planned with the line.
Both lines were derived under 5% O2 and was split in half at the first passage immediately after stable establishment of hESCs, with one half remaining at 5% O2 (WIBR1/2 5%) and the other half shifted to culture under 20% O2 (WIBR1/2 20%). The oxygen concentration under which the line was maintained after the first splitting may be indicated with a superscript. Please see Lengner CJ et al. (2010) Derivation of pre-x inactivation human embryonic stem cell line in physiological oxygen conditions. Cell (In press)."
(No Additional Restrictions)
Whitehead Institute for Biomedical Research
Whitehead Institute for Biomedical Research/Maya Mitalipova