PERFORMANCE OF RADIOIMMUNOASSAYS AND RADIOIODINATIONS

NIH GUIDE, Volume 24, Number 1, January 13, 1995



RFP AVAILABLE:  NICHD-IRP-95-12



P.T. 34



Keywords:

  METHODOLOGIES/PROCEDURES 

  Bioassay 

  RESEARCH RESOURCES 



National Institute of Child Health and Human Development



The National Institute of Child Health and Human Development (NICHD)

has a requirement for immunoassay, bioimmunoassay, and

radioiodination services.  These services will support ongoing

research conducted by NICHD's Intramural Research Program and other

Intramural Investigators of NIH.  The tests will be quantitated

utilizing radioisotopic and fluorescent markers.  Immunoassays will

routinely be developed that are capable of detecting picomole or

femtomole concentrations of antigen.  Custom iodinations will be

performed in support of such assays with 125I using standard

chloramine-T, lactoperoxidase, and Bolton-Hunter reagents, and

electrolytic and iodogen methodologies.  Among the purification

procedures employed will be Sephadex gel, ion exchange, concanavalin-

A, electrophoresis, and high pressure liquid chromatography.

Specific activity, binding efficiency with excess antibody

trichloroacetic acid precipitation, and chromatographic elution

patterns will be included with each preparation.  The performance of

immuno-and in vitro bioimmunoassays for hormones and certain drugs in

human, monkey, rat, and mouse shall be required.  A minimum of 50,000

assays shall be performed for each year of the contract(s).  This

number may be adjusted depending on the number of actual awards.  The

laboratory of the successful Offeror(s) must be certified by the

Nuclear Regulatory Commission.  In addition, the Contractor must be

available for frequent discussions and monthly meetings with the

Project Officer and the Intramural staff.  The RFP will be available

on or about January 20, 1995.



INQUIRIES



All responsible sources are encouraged to submit a proposal.  Several

awards will be considered.  Organizations desiring a copy of the RFP

should send a self-addressed mailing label along with their written

requests to:



Mrs. Dorothy McKelvin

Office of Grants and Contracts

National Institute of Child Health and Human Development

6100 Executive Boulevard, Room 7A07 MSC 7510

Bethesda, MD  20892-7510



.


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