NIH GUIDE, Volume 23, Number 17, May 6, 1994 P.T. 34 Keywords: Cell Lines National Center for Research Resources The National Center for Research Resources supports the American Type Culture Collection (ATCC), which is an international resource to provide scientists with a variety of biological material. This is a notification sent to all recipients of the cell line ATCC-CRL 1593 (U-937) from Freeze lot F-11205. A cellular cross contamination has been discovered and confirmed by PCR and cytogenetic analyses at the ATCC. The inappropriate cell line has not yet been identified, but is human and has a chromosomal mode of 67 plus other properties different from those of U-937. DNA and cell progeny from additional stocks of U-937 are currently under scrutiny. Results will be made public in due course. The ATCC appreciates that this contamination will have caused considerable confusion plus wasted effort in recipients' laboratories and sincerely regrets the problem. The situation emphasizes the critical need for repeated testing of stocks of cells and organisms for purity. Upon request ATCC will provide replacements for all cultures of F-11205 with aliquots of a new lot of U-937 having authenticity confirmed. INQUIRIES Direct programmatic inquiries regarding this resource to: Elaine Young, Ph.D. ATCC Project Officer National Center for Research Resources Westwood Building, Room 854 Bethesda, MD 20892 Telephone: (301) 594-7906 Direct requests for updated information and replacement cultures to: Robert Hay, Ph.D., Head Cell Culture Department American Type Culture Collection 12301 Parklawn Drive Rockville, MD 20852-1776 Telephone: (301) 231-5529 FAX: (301) 770-1848 .
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