NIH GUIDE, Volume 23, Number 14, April 8, 1994



National Institute of Child Health and Human Development

The National Institute of Child Health and Human Development (NICHD)

Transgenic Mouse Development Facility (NTDF) is a research resource

that develops transgenic mice for investigators requiring these

services.  It was established to provide reliable and affordable

transgenic mice primarily to biomedical research grantees.

Specifically, the NTDF supports basic biomedical research by

providing investigators with the following customized services:

o  Analyzing constructs for microinjection.

o  Microinjecting constructs into fertilized one-cell mouse eggs and

reimplanting into pseudopregnant recipient females.

o  Testing potential founders for DNA integration.

o  Producing at least two integration-positive transgenic mice.

o  Fast turnaround time (12-14 weeks)

Applications for services should be requested from the Director,

NTDF.  Completed applications may be submitted anytime to the NICHD

Project Officer for review.  Review is completed within two weeks of

receipt.  All applications are handled confidentially and those

constructs approved are microinjected at the NTDF.  The Investigator

is charged a non-refundable fee of $500 per construct for initial

analysis.  An additional fee of $1500 per construct is assessed if

two or more integration-positive transgenic mice are produced.

The NTDF is supported by a contract award from the National Institute

of Child Health and Human Development, NIH.


Direct programmatic inquiries regarding this research resource to:

Allan Lock, D.V.M.

NTDF Project Officer

National Institute of Child Health and Human Development

Building 6100, Room 4B01

Bethesda, MD  20892

Telephone:  (301) 496-5541

Direct requests for applications and resource inquiries to:

Mark Swanson, Ph.D. or Mr. Frederick Huntress

NICHD Transgenic Mouse Development Facility

303B College Road East

Princeton, NJ  08540

Telephone:  (609) 520-0300, Ext. 183

FAX:  (609) 520-9864


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