NIH GUIDE, Volume 23, Number 7, February 18, 1994




National Heart, Lung, and Blood Institute

The Hypertension and Kidney Diseases Branch, Division of Heart and

Vascular Diseases, National Heart, Lung, and Blood Institute (NHLBI)

has a requirement for the establishment of a large-scale genotyping

service capable of genotyping large numbers of human and mammalian

DNA samples as a cost-effective means to reliably and efficiently

facilitate identification of genes responsible for cardiovascular,

pulmonary and hematologic diseases.  The genotyping service is to

utilize polymerase chain reaction (PCR)-based techniques to provide

highest quality genotypes and operate on a fee-for-service basis.

Automated methods and robotics are required.  The service will not

isolate genomic DNA from tissues and cells, but must provide storage

of the DNA, which will be provided by users of the service.

Genotypic data will be provided to users of this service as

hard-copies (e.g., graphs, photographs, autoradiographs) and on

computer disc, where it must be compiled into simple software

programs for use in more than one of the commonly used personal

computing systems utilized by scientists.  The service is to focus on

production of samples and is expected in subsequent years to increase

production capacity, while reducing the fee-for-service.  Since other

funding mechanisms are currently available to support the development

of new technologies and methodologies to improve genotyping speed,

quality, and cost, no funds will be available for this explicit

purpose in the contract.  However, the service is responsible for

incorporating any applicable, newly developed technologies,

methodologies, and their refinements, into the existing structure in

subsequent years to improve genotyping quality, to decrease costs and

to increase the production of genotypes.  Genotyping projects will be

submitted to the service, wherein a review panel of scientific

experts, established and coordinated by the Contractor, will assess

the scientific merit, feasibility, cost and, in conjunction with the

NHLBI, programmatic relevance.  In order to ensure that the service

has sufficient work, projects outside of NHLBI programmatic relevance

will be considered for genotyping.  However, meritorious projects

with NHLBI programmatic relevance will receive priority over projects

supported through other sources.

The purpose of this contract is to provide seed money for the

establishment of the genotyping service.  It will provide decreasing

funds over a five-year period in order to allow for the service to

become fully established, while maintaining itself by

fee-for-service.  Prospective offerors should have well-recognized

expertise and experience in genomics, molecular genetics,

informatics, robotics, and molecular biology.  In particular,

offerors should have experience in PCR-based methods to map genes.  A

single multi-year incrementally funded award is anticipated.  RFP

NIH-NHLBI-HV-94-14 will be released on or about February 18, 1994.


To receive a copy of the RFP, include three labels self-addressed

with your mailing address and must cite RFP No. NIH-NHLBI-HV-94-14.

Requests for copies of the RFP are to be sent to:

Shari L. Spencer

Contracts Operations Branch

National Heart, Lung, and Blood Institute

Federal Building, Room 4C04

Bethesda, MD  20892


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