LARGE SCALE AUTOMATED DNA SEQUENCING OF HUMAN GENES INVOLVED INNEUROLOGICAL DISORDERS



NIH GUIDE, Volume 22, Number 21, June 11, 1993



RFP AVAILABLE:  NIH-NINDS-93-11



P.T. 34



Keywords:

  Nucleic Acid Sequencing 

  Neurological Disorders 



National Institute of Neurological Disorders and Stroke



The National Institute of Neurological Disorders and Stroke (NINDS),

National Institutes of Health (NIH), has a requirement for the

research and development of highly reproducible, rapid DNA template

production and purification procedures.  DNA must be of sufficient

purity for highly accurate automated DNA sequence analysis.

Additional requirements include screening and amplification of clones

from lambda, cosmid or YAC libraries and biological samples.

Libraries of plasmid subclones are to be made from the larger clones.

Genetic typing with highly informative polymerase chain reaction

(PCR) based markers is another requirement.  Work under the proposed

contract will support the mission objectives of the NINDS Core DNA

Sequencing Facility.  The Facility provides rapid DNA sequencing and

other services to NINDS scientists for use in basic and clinical

neuroscience projects.



Prospective offerors should have expertise and experience in several

areas of molecular biology and nucleic acid biochemistry.  In

particular, offerors shall have experience in DNA isolation and

analysis techniques, basic microbiological techniques, growth of

filamentous bacteriophage, use of restriction endonucleases and DNA

modifying enzymes, bacterial transformation, DNA hybridization, and

use of PCR.



This requirement represents a recompetition of work currently being

performed under NINDS Contract No. NO1-NS-0-2387, with Collaborative

Research, Inc., Waltham, MA.  It is expected that the incumbent

Contractor will recompete.



A single award is anticipated with the performance period not to

exceed three years.



This is not a Request for Proposals (RFP).  RFP No. NIH-NINDS-93-11

will be issued on or about June 11, 1993, with responses due on or

about July 31, 1993.



INQUIRIES



To receive a copy of the RFP, submit a written request and supply two

self-addressed mailing labels to:



Contracts Management Branch

Division of Extramural Activities

National Institute of Neurological Disorders and Stroke

Federal Building, Room 901

7550 Wisconsin Avenue

Bethesda, MD  20892



Reference:  RFP No. NIH-NINDS-93-11



This announcement does not commit the NINDS to make an award.  All

responsible sources may submit a proposal that will be considered by

the Government.



.

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