NICHD TRANSGENIC MOUSE DEVELOPMENT FACILITY

NIH GUIDE, Volume 22, Number 21, June 11, 1993



P.T. 34



Keywords:

  Animal Breed. & Facil., Scientific 

  Genetics 

  Nucleic Acids 



National Institute of Child Health and Human Development



The NICHD Transgenic Mouse Development Facility (NTDF) is a research

resource that develops transgenic mice for investigators requiring

these services.  It was established to provide reliable and

affordable transgenic mice primarily to biomedical research grantees.



Specifically, the NTDF supports basic biomedical research by

providing investigators with the following customized services:



o  Analyzing constructs for microinjection.



o  Microinjecting constructs into fertilized one-cell mouse eggs and

reimplanting into pseudopregnant recipient females.



o  Testing potential founders for DNA integration.



o  Producing at least two integration-positive transgenic mice.



Applications for services should be requested from the Director,

NTDF.  Completed applications are submitted anytime to the NICHD

Project Officer for review.  All applications are handled

confidentially and those constructs approved are microinjected at the

NTDF.  The Investigator is charged a non-refundable fee for initial

analysis of the constructs.  An additional fee is assessed if two or

more integration-positive transgenic mice are produced.



The NTDF is supported by a contract award from the National Institute

of Child Health and Human Development, NIH.



INQUIRIES



Direct programmatic inquiries regarding this research resource to:



Allan Lock, D.V.M.

NTDF Project Officer

National Institute of Child Health and Human Development

Building 6100, Room 4B01

Bethesda, MD  20892

Telephone:  (301) 496-5541



Direct requests for applications and resource inquiries to:



Mark Swanson, Ph.D.

Director, NICHD Transgenic Mouse Development Facility

303B College Road East

Princeton, NJ  08540

Telephone:  (609) 520-0300

FAX:  (609) 520-9864



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