NOTICES OF AVAILABILITY (RFPs AND RFAs)

ONCOGENE ANALYSIS FOR MOLECULAR TOXICOLOGY STUDIES



NIH GUIDE, Volume 21, Number 12, March 27, 1992



RFP AVAILABLE:  NIH-ES-92-25



P.T. 34



Keywords:

  Toxicology 

  Genetics 

  Oncology 

  Nucleic Acid Sequencing 



National Institute of Environmental Health Sciences



The primary purpose of this project will be to provide laboratory

support by the application of assays to screen for and verify mutations

in oncogenes, such as ras or p53, in samples of frozen or fixed tissues

using a variety of molecular techniques.  Specific methods to be in

place include polymerase chain reaction (PCR) amplification,

oligonucleotide hybridization, restriction fragment length polymorphism

(RFLP) analysis, direct sequencing of PCR products, and single stranded

conformation polymorphism (SSCP).  Specific tasks include, but are not

limited to, DNA extraction, amplification of regions of p53 or other

genes, screening for mutational activities of ras or other genes or

mutational deactivation of tumor suppressor genes, and assay for loss

of heterozygosity.  All responsible sources may submit a proposal that

will be considered.  The Request for Proposals (RFP) for this estimated

five-year competitive procurement will be released on or about March

27, 1992.  Proposals will be approximately 40 days thereafter.  The

institute expects to made one award from this solicitation.



Requests must reference RFP NIH-ES-92-25 and must be forwarded to:



National Institute of Environmental Health Sciences

Contracts and Procurement Management Branch

ATTN:  Thomas M. Hardee, Contracting Officer

79 T.W. Alexander Drive, 4401 Building

P. O. Box 12874

Research Triangle Park, NC  27709

Telephone:  (919) 541-7893



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