Release Date: January 16, 2013
National Institute of Mental Health (NIMH)
National Cancer Institute (NCI)
National Eye Institute (NEI)
National Institute on Aging (NIA)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
National Institute on Deafness and Other Communication Disorders (NIDCD)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
National Institute on Drug Abuse (NIDA)
National Institute of General Medical Sciences (NIGMS)
National Institute of Neurological Disorders and Stroke (NINDS)
The purpose of this notice is to facilitate access for researchers to a screening laboratory to develop an High Throughput Screening (HTS) assay and thereby submit a grant application to PAR-12-058 (R01) or PAR-12-059 (R21).
The screening laboratory (e.g., Academic Screening Facilities Directory, http://www.slas.org/screeningFacilities/facilityList.cfm) may provide advice such as identification and selection of commercial HTS assay reagents, and suitable HTS assay format and readout. In addition, the screening laboratory may be able to provide assistance in adapting assays to an HTS format (e.g., 1536-well or 384-well microplate) and performing a pilot screen of a small library of compounds (e.g., the Library of Pharmacologically Active Compounds (LOPAC) collection, the NIH Clinical Collection, etc.) to generate sufficient preliminary assay data to support a grant application submission. Further, the researchers might seek advice from the screening laboratory about orthogonal assays to validate the screening hits, and advice on chemical improvement of the initial hits via structure-activity relationship (SAR) study.
Other technical resources about HTS assay development include the online comprehensive guidebook Assay Guidance Manual (http://www.ncbi.nlm.nih.gov/books/NBK53196/), HTS assay protocols deposited on PubChem BioAssay data base (http://www.ncbi.nlm.nih.gov/sites/entrez?db=pcassay), ASSAY and Drug Development Technologies, a peer-reviewed bimonthly journal, and the Journal of Biomolecular Screening.
Inquiries regarding this Notice may be directed to: