and Human Services (HHS)
BMAP: GENE DISCOVERY IN THE DEVELOPING NERVOUS SYSTEM
Release Date: July 10, 2000
NOTICE: MH-00-011
National Institute of Mental Health
The National Institute of Mental Health proposes to issue solicitation No.
NIMH-00-DB-0006 entitled "BMAP: GENE DISCOVERY IN THE DEVELOPING NERVOUS
SYSTEM " as a 100 percent full and open competitive procedure under SIC Code
8733. The NIMH plans to award one (1) cost-reimbursement completion type
contract, with a three-year base period and two (2) one-year options, on or
about December 23, 2000.
In 1998, the National Institute of Mental Health (NIMH) and the National
Institute of Neurological Disorders and Stroke (NINDS) initiated the Brain
Molecular Anatomy Project (BMAP), an interdisciplinary project to establish
state-of-the-art technologies and informatics systems to decipher the
molecular anatomy of the mammalian brain. As part of this multi-institute
effort, NIMH and NINDS awarded a contract (N01 MH-80014) to the University of
Iowa (M. B. Soares, PI) to support construction of cDNA libraries from 10
brain regions, spinal cord, and retina in the adult mouse using strain
C57BL6/J. This two-year project, which will be completed by the end of
Calendar Year 2000, aims to identify over 75,000 3 ESTs from serially
subtracted libraries and generate a non-redundant collection of more than or
equal to 20,000 mouse brain cDNA clones. The biological resources obtained
through current BMAP contract will be made widely available to the scientific
community. These resources include EST sequences, cDNA clones and libraries,
and a collection of non-redundant arrayed cDNA clones representing
transcripts expressed in adult mouse neural tissue ( BMAP UniGene sets).
This contract is a recompetition and expansion of this work.
The primary goal of this contract is the discovery of novel genes expressed
in the developing mouse nervous system. This contract requires the
production of high-quality, representative cDNA libraries from specific
anatomical regions of the mouse nervous system at different developmental
stages to identify and discover the genes whose expression is restricted
spatially and temporally during development. The utility of this approach
for novel gene discovery, namely utilization of representative cDNA libraries
and determination of Expressed Sequence Tags (ESTs) present in the cDNA
libraries, clearly has been demonstrated by the success of the on-going BMAP
gene discovery project. The primary requirements are: 1) Preparation and
isolation of high-quality RNA using improved and/or new technologies for the
purpose of generating cDNA libraries from the developing mouse nervous
system, 2) Preparation of high-quality representative cDNA libraries using
state-of-the-art techniques (use approved protocols to prepare approximately
twenty (20) cDNA libraries in combination of developmental stages and regions
of the nervous system), 3) Utilization of high-throughput sequencing to
assess the quality of the cDNA libraries, generation of BMAP Developmental
UniGene Sets (perform DNA sequencing on clones in cDNA libraries prepared in
Requirement 2 above, in order to generate up to 100,000-150,000 3" ESTs,
during the 3-year base period of the contract), and 4) Dissemination of data,
storage and distribution of biological materials.
If exercised by the Government, there are options to extend the term of the
contract as follows: each option year following the three-year base contract
period will require the Contractor to prepare eight (8) to twelve (12) cDNA
libraries per year, which will include developmental stages and regions of
the nervous system that are not covered initially. Also, if exercised by the
Government , there are options for increased quantities for the base period
and optional periods, as follows: the Contractor shall, during the base
period, generate up to 15 additional cDNA libraries, and 150,000 EST
sequences, from various related mouse tissues derived from the nervous system
(e.g., sensory systems including visual, olfactory, auditory, and peripheral
nervous system tissues), and during each option period, generate up to 10
additional cDNA libraries, and 100,000 EST sequences, per year from various
related mouse tissues.
There is a mandatory qualification criteria that a statement must be included
in the proposal as follows: 1) the offeror agrees and is committed to
producing materials and data that shall be widely distributed, in a timely
manner, 2) the offeror agrees to provide sequencing data to GenBank at least
every two weeks, and 3) upon request to provide individual cDNA clones and
arrayed cDNA libraries during the contract term and/or at the conclusion of
the contract, to the NIMH or to a third party designated by the NIMH
Request for Proposal (RFP) No. NIMH-00-DB-0006, will be available on or about
July 11, 2000, and the receipt date for proposals will be on or about
September 12, 2000. The RFP may be accessed through the NIMH Home Page by
at:
http://www.nimh.nih.gov/grants/indexcon.cfm
Please note that the RFP for this requirement will include the Statement of
Work, Deliverables and Reporting Requirements, the Technical Evaluation
Criteria, and proposal preparation instructions. All information required
for the submission of an offer will be contained in the electronic RFP
package. Following proposal submission and the initial review process,
offerors included in the competitive range may be requested to provide
additional documentation to the Contracting Officer. All responsible sources
may submit a proposal that will be considered by the NIMH. This
advertisement does not commit the government to award a contract.
INQUIRIES:
Inquiries may be directed to:
Bruce E. Anderson, Contracting Officer
Contracts Management Branch
National Institute of Mental Health, NIH
Neuroscience Center Bldg., Rm. 6107 (MSC 9603)
Bethesda, MD 20892-9603
Telephone: (301) 443-2234
Fax: (301) 443-0501
Email: [email protected]
Weekly TOC for this Announcement
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