Full Text DC-96-005 MOLECULAR AND CELLULAR PATHOLOGY OF THE HUMAN AUDITORY AND VESTIBULAR SYSTEMS NIH GUIDE, Volume 25, Number 19, June 14, 1996 RFA: DC-96-005 P.T. 34 Keywords: Pathogenesis Gene Cloning Biology, Cellular Biology, Molecular Molecular Genetics National Institute on Deafness and Other Communication Disorders Letter of Intent Receipt Date: September 17, 1996 Application Receipt Date: October 17, 1996 PURPOSE The National Institute on Deafness and Other Communication Disorders (NIDCD) of the National Institutes of Health (NIH) invites grant applications to support cellular biologic, molecular biologic, and molecular genetic studies of newly acquired and archival human temporal bones and associated brain tissues of individuals who had well-documented evidence of disorders of hearing and/or balance before death. It is believed that studies of this kind can lead to a better understanding of the pathogenesis and pathophysiology of disorders of the human auditory and vestibular systems. HEALTHY PEOPLE 2000 The Public Health Service (PHS) is committed to achieving the health promotion and disease prevention objectives of "Healthy People 2000," a PHS-led national activity for setting priority areas. This RFA, Molecular and Cellular Pathology of the Human Auditory and Vestibular Systems, is related to the priority areas of physical activity fitness, unintentional injuries, diabetes and chronic disabling diseases and clinical prevention services. Potential applicants may obtain a copy of "Healthy People 2000" (Full Report: Stock No. 017-001-11474-0 or Summary Report: Stock No. 017-001-11473-1) through the Superintendent of Documents, Government Printing Office, Washington, DC 20402-9325 (telephone 202-512-1800). ELIGIBILITY REQUIREMENTS Applications may be submitted by domestic and foreign for-profit and non-profit organizations, public and private, such as universities, colleges, hospitals, laboratories, units of State and local governments, and eligible agencies of the Federal government. Foreign institutions are not eligible for First Independent Research Support Transition (FIRST) (R29) awards. Consortia arrangements are encouraged. Domestic applications may include international components. Racial/ethnic minority individuals, women and persons with disabilities are encouraged to apply as Principal Investigators. MECHANISM OF SUPPORT This RFA will use the National Institutes of Health (NIH) individual research project grant (R01) and the FIRST (R29) award. The total project period for an application submitted in response to this RFA may not exceed five years. Responsibility for the planning, direction, and execution of the proposed project will be solely that of the applicant. This RFA is a one-time solicitation, with an anticipated award date of August 1, 1997. Future applications for the competing continuation of successful responses to this RFA will compete with all investigator-initiated applications and be reviewed according to the customary peer review procedures. Applicants from institutions that have a General Clinical Research Center (GCRC) funded by the NIH National Center for Research Resources (NCRR) may wish to identify the GCRC as a resource for conducting the proposed research. If so, a letter of agreement from either the GCRC program director or Principal Investigator should be included with the application. FUNDS AVAILABLE It is expected that $750,000 will be available for the first year of support (direct cost) for this RFA and that up to three applications will be funded. The level of support will be dependent upon the scientific merit and scope of the applications and the availability of funds. RESEARCH OBJECTIVES Background Studies of the histochemistry, immunohistochemistry and histopathology of the human temporal bone and associated brain structures have provided valuable knowledge about disorders of hearing and balance. Techniques that enable antigen retrieval and DNA extraction from routinely processed archival temporal bones have been developed recently. These technical advances provide additional opportunity for investigating the agents of disease in the auditory and vestibular systems at the cellular and molecular levels. Temporal bone collections with well-documented clinical histories have thus become invaluable sources of proteins and DNA for the study of auditory and vestibular pathology. Several reports of the successful extraction of RNA and DNA from archival human temporal bone sections and subsequent amplification by polymerase chain reaction (PCR) have been published. This methodology enables the identification of genetic mutations and viral nucleic acid sequences in pathologic tissues. These studies have provided the first identification and location of varicella-zoster virus DNA in herpes zoster oticus and of herpes simplex virus DNA in Bell's palsy. Recently, reverse transcription of extracted RNA followed by PCR amplification enabled the identification of a 115-base-pair sequence of the measles virus in a series of temporal bones with histologic evidence of otosclerosis. This study provides evidence for a possible role of the measles virus in the etiology of otosclerosis. In order to advance histopathologic study of the human temporal bone, particularly in the directions that are the focus of the present RFA, the NIDCD National Temporal Bone, Hearing and Balance Pathology Resource Registry was established by the NIDCD in 1992 under a contract awarded to the Massachusetts Eye and Ear Infirmary. The Registry serves as a national resource, cataloging the pathologic material in all known temporal bone collections in the United States and enabling investigators to identify and locate this material by clinical and histopathologic diagnoses and by other data so that further studies may be pursued. To date, the Registry contains information on 6,227 individuals (approximately 11,500 specimens) held in twenty-six facilities, including all fourteen laboratories known to be currently active in temporal bone research. The Registry is directly accessible to remote- site users in member laboratories and to other researchers directing requests for data searches to the Registry staff. Other functions and accomplishments of the Registry are described in a journal article (Arch Otolaryngol Head Neck Surg, 1993; 119: 856-853) and in the semiannual newsletter published by the Registry. Scope The characterization of specific genetic alterations, viral infections and other etiologic agents of disease using the techniques of cellular and molecular biology holds great promise in advancing our understanding of auditory and vestibular disorders. There are emerging opportunities to apply these techniques to newly acquired and archival temporal bone and brain tissue specimens, thus enabling the investigator to relate the pathogenesis, pathophysiology and staging of the disease. This initiative seeks to foster prospective, hypothesis-driven investigations of the pathogenesis and pathophysiology of diseases and disorders of the auditory system and/or the vestibular system using the techniques of cellular biology (e.g., immunohistochemistry) and/or molecular biology (e.g., subtractive hybridization, in situ hybridization, PCR, reverse transcriptase PCR, in situ PCR) in newly acquired and archival human temporal bone and associated brain tissues. Understanding the viral, genetic, immune system-mediated and other bases of these diseases is a prerequisite to the development of novel strategies for prophylaxis and therapeutics. Examples of studies utilizing newly acquired and archival human temporal bone and associated brain tissue specimens that would be responsive to this RFA include, but are not limited to: o application of the techniques of genome analysis, such as linkage analysis and positional cloning, to individuals who have been studied clinically for hereditary hearing or balance impairments; o characterization of mutations in the mitochondrial or nuclear genome as predisposing or causal factors in auditory or vestibular system disorders, such as presbycusis, noise- induced hearing loss and ototoxicity; o studies of immune cells, inflammatory mediators and other molecules in chronic infectious or immune system-mediated disorders of the auditory or vestibular systems; o analyses of the molecular and cellular pathology associated with idiopathic sudden sensorineural hearing loss; and o searches for viral or bacterial genomes to provide evidence for the causal or contributory roles of these microorganisms in auditory or vestibular disorders, such as otosclerosis and Meniere's disease. INCLUSION OF WOMEN AND MINORITIES IN RESEARCH INVOLVING HUMAN SUBJECTS It is the policy of the NIH that women and members of minority groups and their subpopulations must be included in all NIH supported biomedical and behavioral research projects involving human subjects, unless a clear and compelling rationale and justification is provided that inclusion is inappropriate with respect to the health of the subjects or the purpose of the research. This new policy results from the NIH Revitalization Act of 1993 (Section 492B of Public Law 103-43) and supersedes and strengthens the previous policies (concerning the Inclusion of Women in Study Populations, and concerning the Inclusion of Minorities in Study Populations), which have been in effect since 1990. The new policy contains some provisions that are substantially different from the 1990 policies. All investigators proposing research involving human subjects should read the NIH Guidelines For Inclusion of Women and Minorities as Subjects in Clinical Research, which was published in the Federal Register of March 28, 1994 (FR 59 14508-14513) and reprinted in the NIH Guide For Grants and Contracts, Volume 23, Number 11, March 18, 1994. Investigators also may obtain copies of the policy from the program staff listed under INQUIRIES. Program staff may also provide additional relevant information concerning the policy. LETTER OF INTENT Prospective applicants are asked to submit, by September 17, 1996, a letter of intent that includes a descriptive title of the proposed research, the name, address, and telephone number of the Principal Investigator, the identities of other key personnel and participating institutions, and the number and title of the RFA in response to which the application may be submitted. Although a letter of intent is not required, is not binding, and does not enter into the review of subsequent applications, it is helpful in planning for the review of applications. It allows Institute staff to estimate the potential review workload and to avoid conflict of interest in the review. The letter of intent is to be sent to: Acting Chief, Scientific Review Branch National Institute on Deafness and Other Communication Disorders Executive Plaza South, Room 400C 6120 Executive Boulevard MSC-7180 Bethesda, MD 20892-7180 Telephone: (301) 496-8683 FAX: (301) 402-6250 APPLICATION PROCEDURES The research grant application form PHS 398 (rev. 5/95) is to be used in applying for these grants. Applications kits are available at most institutional offices of sponsored research and may be obtained from the Office of Extramural Outreach and Information Resources, National Institutes of Health, 6701 Rockledge Drive, MSC 7910, Bethesda, MD 20892-7910, telephone 301/710-0267, email: ASKNIH@odrockm1.od.nih.gov; and from the program administrator listed under INQUIRIES. The PHS 398 form is also available electronically on the NIH Home Page at https://grants.nih.gov/grants/phs398. The RFA label available in the PHS 398 (rev. 5/95) application form must be affixed to the bottom of the face page of the application. Failure to use this label could result in delayed processing of the application such that it may not reach the review committee in time for review. In addition, the RFA title and number must be typed on line 2 of the face page of the application form and the YES box must be marked. Submit a signed, typewritten original of the application, including the Checklist, and three signed photocopies, in one package to: DIVISION OF RESEARCH GRANTS NATIONAL INSTITUTES OF HEALTH 6701 ROCKLEDGE DRIVE, ROOM 1040 - MSC 7710 BETHESDA, MD 20892-7710 BETHESDA, MD 20817 (for courier/overnight service) At the time of submission, two additional copies of the application must be sent to the Chief, Scientific Review Branch, at the address listed under LETTER OF INTENT. Applications must be received by October 17, 1996. If an application is received after that date, it will be returned to the applicant without review. The Division of Research Grants (DRG) will not accept any application in response to this RFA that is essentially the same as one currently pending initial review, unless the applicant withdraws the pending application. The DRG will not accept any application that is essentially the same as one already reviewed. This does not preclude the submission of substantial revisions of applications already reviewed, but such applications must include an introduction addressing the previous critique. REVIEW CONSIDERATIONS Upon receipt, applications will be reviewed for completeness by DRG and for responsiveness by the NIDCD. Incomplete applications will be returned to the applicant without further consideration. If the application is not responsive to the RFA, NIDCD program staff may contact the applicant to determine whether to return the application to the applicant or submit it for review in competition with unsolicited applications at the next review cycle. Applications that are complete and responsive to the RFA will be evaluated for scientific and technical merit by an appropriate peer review group convened by the NIDCD in accordance with the criteria stated below. As part of the initial merit review, all accepted applications will receive a written critique and may undergo a process in which only those applications deemed to have the highest scientific merit, generally the top half of all applications under review, will be discussed, assigned a priority score, and receive a second level review by the National Deafness and Other Communication Disorders Advisory Council. Review criteria for this RFA are in general the same as those for traditional research grant applications: o scientific or technical merit and originality of proposed research; o appropriateness and adequacy of the experimental approach and methodology proposed to carry out the research; o qualifications and research experience of the Principal Investigator and the other personnel proposed to carry out the research; o availability of resources necessary to perform the research; o appropriateness of the proposed budget and duration in relation to the proposed research; and o responsiveness of the application to the purpose of this RFA. AWARD CRITERIA The following factors will be considered in making award decisions: o Scientific and technical merit, as determined by peer review; o Programmatic priorities; and o Availability of funds. INQUIRIES Inquiries concerning this RFA are encouraged. The opportunity to clarify any issues or questions from potential applicants is welcome. Direct inquiries regarding scientific and programmatic issues to: Daniel A. Sklare, Ph.D. Division of Human Communication National Institute on Deafness and Other Communication Disorders Executive Plaza South, Room 400C 6120 Executive Boulevard MSC 7180 Bethesda, MD 20892-7180 Telephone: (301) 496-1804 FAX: (301) 402-6251 Email: daniel_sklare@nih.gov Direct inquiries regarding fiscal matters to: Sharon Hunt Division of Extramural Activities National Institute on Deafness and Other Communication Disorders Executive Plaza South, Room 400B 6120 Executive Boulevard MSC 7180 Bethesda, MD 20892-7180 Telephone: (301) 402-0909 FAX: (301) 402-1758 Email: SH79F@nih.gov AUTHORITY AND REGULATIONS This program is described in the Catalog of Federal Domestic Assistance No. 93.173. Awards are made under authorization of the Public Health Service (PHS) Act, Title IV, Part A (Public Law 78-410, as amended by Public Law 99-158, 42 USC 241 and 285) and administered under PHS grants policies and Federal Regulations 42 CFR 52 and 45 CFR Part 74. This program is not subject to the intergovernmental review requirements of Executive Order 12372 or Health Systems Agency review. The PHS strongly encourages all grant and contract recipients to provide a smoke-free workplace and promote the non-use of all tobacco products. In addition, Public Law 103-227, the Pro-Children Act of 1994, prohibits smoking in certain facilities (or in some cases, any portion of a facility) in which regular or routine education, library, day care, health care or early childhood development services are provided to children. This is consistent with the PHS mission to protect and advance the physical and mental health of the American people. .
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