NIH GUIDE, Volume 25, Number 29, August 30, 1996




P.T. 34


  Nucleic Acid Sequencing 

  Neurological Disorders 


National Institute of Neurological Disorders and Stroke


The NINDS has a requirement for the continued development of highly

reproducible rapid DNA template production and purification

procedures that provide DNA of sufficient purity for highly accurate

automated DNA sequence analysis and significantly improve techniques

currently used for genotyping and positional cloning.  Additional

requirements include screening and amplification of clones from

lambda, cosmid, or YAC libraries and biological samples.  Libraries

of plasmid subclones are to be made from the larger clones.  Genetic

typing with highly informative PCR based markers shall also be

required.  Work under the proposed contract will support the

objectives of the NINDS Core DNA Facility.  The NINDS Facility

provides rapid DNA sequencing and other types of DNA analysis to

NINDS scientists for use in Basic Neuroscience and Clinical

Neurogenetics projects.  The Contractor will be required to provide

approximately 5,000 purified DNA template preparations/year by

generating nested deletions in clones of interest; perform genotyping

in the amount of approximately 300 markers per 200 DNA samples/year;

and perform screening of approximately 10 libraries.  The sources of

DNA shall be provided by the Government.  The NINDS DNA Facility will

be responsible for evaluation of materials and results.  Prospective

offerors must have staff with expertise and experience in molecular

biology, nucleic acid biochemistry, computer analysis and robotics;

and operational experience in large scale DNA isolation and analysis

techniques, basic microbiological techniques, use of DNA modifying

enzymes, bacterial transformation, DNA hybridization and use of

polymerase chain reaction (PCR).  This is a recompetition or work

currently being performed under Contract NO1-NS-4-2305, with Genome

Therapeutics Corporation, Waltham, MA.  The Government anticipates

awarding one contract with a performance period of five years.  RFP

No. NIH-NINDS-96-13 will be issued on or about September 6, 1996,

with a tentative closing date for receipt of proposals of October 31,





All responsible sources are encouraged to submit a proposal that will

be considered by the NINDS.  To receive a copy of the RFP, submit a

written request and two self-addressed mailing labels to:


Kirkland L. Davis

Contracts Management Branch

National Institute of Neurological Disorders and Stroke

7550 Wisconsin Avenue, Room 901 - MSC 9190

Bethesda, MD  20892-9190




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