HIGH THROUGHPUT ANALYSIS OF GENE EXPRESSION PATTERNS IN THE NERVOUS SYSTEM

Release Date:  January 11, 2000

NOTICE:  NS-00-004

RFP Available: NIH-NINDS-RFP-00-05 

National Institute of Neurological Disorders and Stroke 

The National Institute of Neurological Disorders and Stroke (NINDS), National 
Institutes of Health, is committed to research to promote the understanding of gene 
function in the nervous system.  Once all the genes of the human genome have been 
sequenced, the next great challenge for biomedical research will be to unravel the 
functions of these genes.  A crucial step in this process will be to analyze their 
spatial expression patterns, and to determine how these patterns change under 
different developmental and physiological conditions.  In the past, analyses of gene 
expression patterns have been done on a gene-by-gene basis within individual 
laboratories.  Such analyses generally focus on a small number of anatomical regions 
that are of particular interest to the investigator doing the analysis, and are of 
limited value to the community as a whole:  that is, researchers wishing to know a 
gene’s expression pattern in their own anatomical region of interest are forced to 
redo the analysis in their own laboratories.  Thus, a general reference atlas of 
gene expression patterns in the nervous system would be of great value to the 
neuroscience community.  Based on these considerations, the NINDS has decided to 
establish a public database of gene expression patterns for the nervous system.  
This project will involve screening probes for a large number (thousands) of gene 
products on a relatively limited number (10 to 20) of sections of the nervous system 
cut through standardized planes of section.  Planes of section will be chosen to 
include the structures of most general interest to the neuroscience community (for 
example, cortex, basal ganglia, hippocampus, and the spinal cord), so as to maximize 
the utility of the data for the greatest number of investigators.  The resulting 
images of gene expression will be digitized and entered into a Web-accessible 
database.  The initial phase of the project will focus on adult mice and mice at 
three stages of development (e.g., early and late embryonic and early postnatal).  
Later stages of the project will add data for mutant mice subject to specific 
experimental manipulations.  No human studies are required.

Personnel with established expertise in histological processing of nervous tissue to 
analyze gene expression patterns, mouse genetics, neuroanatomy, histology, and 
embryology will be needed to perform this research.  

It is anticipated that one award may be made for a period of three years (plus two 
one-year option periods) in September 2000.  

Request for Proposals (RFP) NIH-NINDS-00-05 will be AVAILABLE ELECTRONICALLY ONLY.  
It will be available at this website 
(http://www.ninds.nih.gov/funding/areas/contracts_management_branch/index.htm)
 15 or more calendar days after the issuance of this synopsis.  OFFERORS ARE 
 RESPONSIBLE FOR ROUTINELY CHECKING THIS WEBSITE FOR ANY POSSIBLE SOLICITATION 
 AMENDMENTS THAT MAY BE ISSUED.  NO INDIVIDUAL NOTIFICATION OF ANY AMENDMENTS 
 WILL BE PROVIDED.  All responsible sources may submit a proposal which shall 
 be considered by the agency.  Refer to numbered note 26.****

INQUIRIES

Inquiries may be directed to:

Laurie A. Leonard, Contracting Officer
Contracts Management Branch
National Institute of Neurological Disorders and Stroke
Neuroscience Center, Room 3287
6001 Executive Boulevard, MSC 9531
Bethesda, MD  20892-9531
Tel:  (301) 496-1813
Fax:  (301) 402-4225
Email:  LL44S@nih.gov



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